Measurement of N, the number of 14 C atoms currently in the sample, allows the calculation of t, the age of the sample, using the equation above. The above calculations make several assumptions, such as that the level of 14 C in the atmosphere has remained constant over time. The calculations involve several steps and include an intermediate value called the “radiocarbon age”, which is the age in “radiocarbon years” of the sample: Radiocarbon ages are still calculated using this half-life, and are known as “Conventional Radiocarbon Age”. Since the calibration curve IntCal also reports past atmospheric 14 C concentration using this conventional age, any conventional ages calibrated against the IntCal curve will produce a correct calibrated age. When a date is quoted, the reader should be aware that if it is an uncalibrated date a term used for dates given in radiocarbon years it may differ substantially from the best estimate of the actual calendar date, both because it uses the wrong value for the half-life of 14 C, and because no correction calibration has been applied for the historical variation of 14 C in the atmosphere over time. The different elements of the carbon exchange reservoir vary in how much carbon they store, and in how long it takes for the 14 C generated by cosmic rays to fully mix with them. This affects the ratio of 14 C to 12 C in the different reservoirs, and hence the radiocarbon ages of samples that originated in each reservoir.
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We are constantly evaluating the utility of given probe sets and probe designs, in addition to expanding the number of UCE loci we are targeting. We have several larger probes sets in the works, and we are also working on optimizing probe sets based on their capture success, phylogenetic utility, etc. Please check back for updates. You can now buy each of these probe sets direct from MYcroarray in the form of a capture kit.
MYcroarray has even made a discounted “pilot” sized kit available for labs who want to do some test enrichments. We used these probes for our in-silico analysis of the placental mammal phylogeny, our in vitro analysis of extant bird groups, and our in vitro analysis of the phylogenetic position of turtles. By their deposition in Dryad, all probes are available under a CC0 license , thus freely available for you to use, without restriction. We designed probes from UCEs by including flanking sequence from chickens.
Because of the highly conserved nature of UCEs and their flanking sequence, we have found these probes work well across amniotes. We used these probes for our in-silico analysis of the primate phylogeny, and the 2, probes targeting 2, loci are a subset of this larger set of probes. All probes are available under a CC0 license , thus freely available for you to use, without restriction.
Although this probe set is not, yet, referenced in a publication, we have been using it for some time across a variety of taxa with much success. Below is the probe design that we used to understand relationships among the early diverging teleosts Faircloth et al. We have several larger probes sets in the works for fishes, and we are also working on optimizing probe sets based on their capture success, phylogenetic utility, etc.